Flow cytometry- It is used to measure the number of cells from a suspension of heterogenous population. It can analyze and sort particles on a one by one basis at rates of 50
000 particles per second. It consist of 3 main components-
1.The flow system
2.The optical system
3.The electronic system
Flow system- The sample is injected into the centre of a stream of liquid (water or buffer), called the sheath fluid. the particles are randomly distributed.
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000 particles per second. It consist of 3 main components-1.The flow system
2.The optical system
3.The electronic system
Flow system- The sample is injected into the centre of a stream of liquid (water or buffer), called the sheath fluid. the particles are randomly distributed.
The optical system-
After hydrodynamic focusing, each particle passes through one or more beams of light(lasers).Light scattering or fluorescence emission (assumed the particle is labeled by a fluorochrome) provides information about the particle’s properties. Fluorescence measurements taken at different wavelengths can provide quantitative and qualitative data about fluorochrome-labeled cell surface receptors or intracellular molecules such as DNA and cytokines. Light is quantified by detectors.The specificity of detection is controlled by optical filters, which block certain wavelengths while transmitting others. Charged droplets are deflected electrostatically by passage through an electrical field.
After hydrodynamic focusing, each particle passes through one or more beams of light(lasers).Light scattering or fluorescence emission (assumed the particle is labeled by a fluorochrome) provides information about the particle’s properties. Fluorescence measurements taken at different wavelengths can provide quantitative and qualitative data about fluorochrome-labeled cell surface receptors or intracellular molecules such as DNA and cytokines. Light is quantified by detectors.The specificity of detection is controlled by optical filters, which block certain wavelengths while transmitting others. Charged droplets are deflected electrostatically by passage through an electrical field.
The electronic system- It analyze statistically by software to report cellular components, size,phenotype .
Applications of Flow Cytometry-
Typical applications of flow cytometry include-
- Biomolecular studies
- Immunology
- Single cell analysis
- Medical applications (hematology, genetics)
- Autofluorescence characterization.
1.Flow cytometer is used to measure the number of-
A. Cells c. Proteins
B. Dna d. Rna
Ans. A (cells)
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