PAPAIN AND PEPSIN DIGESTION OF IMMUNOLOGY

MCQs-
1.Papain digest IgG into-
A. two Fab fragments and one Fc fragment
B. three Fab fragments and two Fc fragments
C. two Fab fragments and two Fc fragments
D. three Fab fragments and three Fc fragments
Ans.A

2. Papain is found in
A. papaya
B. proteases
C. pepsin
D. pancreatic amylase
Ans. 1

3.Which enzymres are in the composition of gastric juice ?

(A) Ptylin, Renin, Lipase
 (B) Pepsin, Ptylin, Renin
(C) Lipase Pepsin, Renin
(D) Ptylin, Lipase,Pepsin
Ans. C
4.Pepsin has an optimum pH of:
a) 1
b) 1.2
c) 1.4
d) 1.6
Ans. d
Note-Enzyme papain cleaves the immunoglobulin at the N-terminal side of the inter-heavy chain disulfide bond and produces three fragments (two Fab fragments and one Fc fragment).

Enzyme pepsin cleaves the immunoglobulin at the C-terminal region and produces one large fragment (which contains two Fab regions) called F (ab’) 2. The remaining heavy chains on the C-terminal side are digested into multiple small peptides.

4. Cleavage of IgG by papain produces:

 A  Divalent antigen binding fragments.
 B  Isolated light chains.
 C  F(ab')2.
D  Fab.

Ans D  Papain splits the hinge sequences above the inter-heavy chain disulfide bond and produces a monovalent antigen binding fragment consisting of light chain and part of the heavy chain containing the VH and CH1 domains.

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