Edman degradation

Edman degradation -(Pher Edman)

Definition-It is a method to determine the sequence of peptides  and proteins from the N- terminal of a polypeptide chain.
Reagent-  Phenylisothiocyanate

Mechanism-

 1.Under alkaline condition-

• Phenylisothiocyanite reacts with N-terminal of amino group of polypeptide .
• The product of the above reaction is phenylthiocarbamyl-peptide derivative (PTC-peptide.)

2. Under acidic condition-

• In the presence of trifluroacetic acid this product cleaved as a thiazolinone derivative.
• It then form phenylthiohydantoin which can be easily identified by using chromatography or electrophoresis.

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