S1 nuclease is an endonuclease which is specific for hydrolyzing single-stranded RNA or DNA molecules. (zinc-requiring ) It is more active on DNA then RNA. The protein is thermostable and resistant to several denaturing agents, such as urea, SDS, and formamide .
m-RNA is hybridized to ss DNA( radiolabelled with a probe ) that overlaps the start of the target transcript . It results in DNA-RNA hybrid. The hybrid molecule has single stranded extensions that are degraded by the single stranded specific S1 nuclease . The 3' end of DNA fragment has been removed by using electrophoresis .
Thank you for visiting my blog.Please feel free to share your comment on this article ,Please subscribe and share the articles to get more such articles.
No comments:
Post a Comment