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Friday, 17 June 2016
CYBRIDS
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Cybrids - It is also called as cytoplasmic hybrid. It consist of 2 protoplast one is donor and other is recipient the nuclear cont...
Thursday, 9 June 2016
S1 nuclease mapping
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S1 nuclease mapping - S1 nuclease is an endonuclease which is specific for hydrolyzing single-stranded RNA or DNA molecules. (...
Tuesday, 7 June 2016
Melting temperature (Tm)
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(Melting temp.-Tm) The temp. at which half the strands of DNA are single stranded and half are double stranded. Half of the DNA are ...
Tuesday, 31 May 2016
Blue white screening
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Blue white screening - Foriegn DNA and plasmid Dna are cut with restriction enzyme. Plasmid has gene for lactose hydrolysis lac z gene w...
Linkers and adapters
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Linkers - Short stretches of dsDNA of known nucloetide sequence (length 8-14 bp )and have a recognition site for 3-8 restriction enzymes. ...
Monday, 30 May 2016
Bioscience formulae
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Bioscience formulae- 1.V P =V G +V E +V GE 2. Crossover value by the square root of the number of individuals (.\/N) 3. Chi-Square Fo...
Pleiotrophy
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Pleiotrophy - One gene have the ability to produce two or more unrelated phenotypic traits. An example is phenylketonuria . The dise...
Saturday, 28 May 2016
Flow cytometry
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Flow cytometry - It is used to measure the number of cells from a suspension of heterogenous population. It c an analyze and sort particles...
Friday, 27 May 2016
Promiscuous dna
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Promiscuous dna - Endosymbiotic transfer of DNA from cytoplasmic organelles (mitochondia and chloroplast) to the nucleus. It is supposed...
Thursday, 26 May 2016
Flippase /Floppase /Scramblase
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1. Flippase(P-type ATpase ) - Transporters that move lipids to the cytoplasmic face of the membrane are commonly called “flippases. It us...
Wednesday, 25 May 2016
Lipid rafts
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Lipid rafts - The plasma membrane is composed of a lipid bilayer inserted various proteins(transmembrane ion-channel and transporter prot...
Cloning
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Cloning: (Dolly) Definition - Clones are organisms that are exact genetic copies. Every single bit of DNA is identical. It can h...
Problems related to hardy weinberg law
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1 . If 98 out of 200 individuals in a population express the recessive phenotype, what percent of the population would you predict would ...
Hardy weinberg equilibrium
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Hardy Weinberg law or Equilibrium by G. H. Hardy (a mathematician) and Wilhelm Weinberg (a physician). “The relative frequencies of ge...
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